Dr. Richard Harvey
Pharmazeutische Technologie und Biopharmazie
Arbeitsgruppe Biopharmazie
Raum 419.2
Kurt-Mothes-Straße 3
06120 Halle (Saale)
Telefon: +49 (0) 345 55-25215
E-Mail: richard.harvey@pharmazie.uni-halle.de
Research Projects
My research has taken me on a bit of a strange journey, from studying the effects of environmental stress on fungal spore germination, via the neurohormonal control of feeding in Locusts to the aggregation behaviour of natural and synthetic lipids and surfactants. I am currently focused on studying molecular interactions between components of the bacterial cell envelope such as lipopolysaccharide and plasma membrane lipids, and membrane-active antimicrobial substances. To achieve this, my group uses a number of different experimental methods ranging from lipidomics and molecular spectroscopy to surface science techniques such as neutron reflectometry and diffraction. The overall aim of this work is to gain insight into physical mechanisms of drug tolerance in multi-resistant bacteria of clinical relevance, to aid the development of future anti-infectives.
Previous Positions
Lecturer in Molecular Pharmaceutics, King’s College London. 2004-2016
Postdoctoral Research Associate, King’s College London. 2003-2004
Postdoctoral Research Associate, Université Paris Sud. 2002-2003
Lecturer, Esher College. 1995-1998
Science Teacher. 1990-1995
NHS Biomedical Scientist. 1988-1989
Education
PhD in Pharmaceutical Biophysics, King’s College London. 1998-2002
MSc Comparative Physiology, Birkbeck College London. 1995-1997
PGCE Secondary Science, Cardiff University. 1989-1990
BSc (Hons) Microbiology, Cardiff University. 1985-1988
Recent Publications
Rehal, R. Gaffney, P.R.J, Hubbard, A.T.M., Barker, R.D. & Harvey, R.D. (2019) The pH-dependence of lipid-mediated antimicrobial peptide resistance in a model staphylococcal plasma membrane: A two-for-one mechanism of epithelial defence circumvention. European Journal of Pharmaceutical Sciences 128: 43-53
Marbach, H., Vizcay-Barrena, G., Memarzadeh, K., Otter, J.A., Pathak, S., Allaker, R.P., Harvey, R.D. & Edgeworth, J.D. (2019) Tolerance of MRSA ST239-TW to chlorhexidine-based decolonization: Evidence for keratinocyte invasion as a mechanism of biocide evasion. Journal of Infection doi.org/10.1016/j.jinf.2018.10.007
Schmid, M., Wölk, C., Giselbrecht, J., Chan, K.L.A. & Harvey, R.D. (2018) A combined FTIR and DSC study on the bilayer-stabilising effect of electrostatic interactions in ion paired lipids. Colloids and Surfaces B: Biointerfaces 169: 298-304
Terakosolphan, W., Trick, J.L., Royall, P.G., Rogers, S.E., Lamberti, O., Lorenz, C.D., Forbes, B. & Harvey, R.D. (2018) Glycerol solvates DPPC headgroups and localises in the interfacial regions of model pulmonary interfaces altering bilayer structure. Langmuir 34: 6941–6954
Urbano, L., Clifton, L., Ku, H.K., Kendall-Troughton, H., Vandera, K.-K.A., Matarese, B.F.E., Abelha, T., Li, P., Desai, T., Dreiss, C.A., Barker, R.D., Green, M.A., Dailey, L.A. & Harvey, R.D. (2018) Influence of the surfactant structure on photoluminescent π-conjugated polymer nanoparticles: interfacial properties and protein binding. Langmuir 34: 6125-6137
Hubbard, A.T.M., Coates, A.R.M. & Harvey, R.D. (2017) Comparing the action of HT61 and chlorhexidine on natural and model Staphylococcus aureusmembranes. Journal of Antibiotics 70: 1020-1025
Rehal, R.P., Marbach, H., Hubbard, A.T.M., Sacranie, A.A., Sebastiani, F., Fragneto, G. & Harvey, R.D. (2017) The influence of mild acidity on lysyl-phosphatidylglycerol biosynthesis and lipid membrane physico-chemical properties in methicillin-resistant Staphylococcus aureus. Chemistry and Physics of Lipids 206: 60-70
Hubbard, A.T.M., Barker, R., Rehal, R., Vandera, K.-K.A., Harvey, R.D. & Coates, A.R.M. (2017) Mechanism of action of a membrane-active quinoline-based antimicrobial on natural and model bacterial membranes. Biochemistry 56: 1163-1174
The influence of rough lipopolysaccharide structure on molecular interactions with mammalian antimicrobial peptides. G Bello, A Bodin, MJ Lawrence, D Barlow, AJ Mason, RD Barker, RD Harvey. Biochimica et Biophysica Acta (2016) 1858; 197–209.
Characterization of the aggregates formed by various bacterial lipopolysaccharides in solution and upon interaction with antimicrobial peptides. G Bello, J Eriksson, AE Terry, K Edwards, MJ Lawrence, DJ Barlow, RD Harvey. Langmuir (2015) 31; 741−751.
Stabilisation of distearoylphosphatidylcholine lamellar phases in propylene glycol using cholesterol. RD Harvey, N Ara, RK Heenan, DJ Barlow, PJ Quinn, MJ Lawrence. Molecular Pharmaceutics (2013) 10; 4408−4417.
Diffraction studies on natural and model lipid bilayers. F Sebastiani, R Harvey, S Khanniche, JB Artero, M Haertlein, G Fragneto. European Physical Journal-Special Topics (2012) 213; 355-365.
Cyclic antimicrobial R-, W-rich peptides: the role of peptide structure and E. coli outer and inner membranes in activity and the mode of action. C Junkes, RD Harvey, KD Bruce, R Dölling, M Bagheri, M Dathe. European Biophysics Journal (2011) 40; 515-528.